EGFR Knockout cell line (HEK293)

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EGFR Knockout cell line (HEK293) Order

EGFR Knockout cell line (HEK293)
Catalog#
YKO-HS5466
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
EGFR Knockout cell line (HEK293)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
EGFR Knockout cell line (HEK293)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
EGFR
Gene ID
1956
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Accession
CVCL_D9E0(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript EGFR-201)
Validation: PCR+Sanger Sequencing
EGFR Knockout cell line (HEK293) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HEK293		 STR Info (Cell bank)
Cell Line:HEK-293.2sus
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 8888
D13S317 12141214
D7S820 11121112
D16S539 913913
VWA 16191619
TH01 79.379.3
AMEL XXXX
TPOX 11111111
CSF1PO 12121212
D12S391 1921
FGA 2323
D2S1338 1919
D21S11 2830.2
D18S51 1718
D8S1179 1214
D3S1358 1517
D6S1043 1111
PENTAE 715
D19S433 1818
PENTAD 910
D1S1656 1517.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the EGFR gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, EGFR gene knockout EGFR Knockout cell line (HEK293) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
EGFR Knockout cell line (HEK293)
EGFR Knockout cell line (HEK293)
Catalog#
YKO-HS5466
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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